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オヤマダ マサヒト
Oyamada Masahito
小山田 正人 所属 藤女子大学 人間生活学部 食物栄養学科 職種 特任教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2008/01 |
| 形態種別 | 研究論文（学術雑誌） |
| 査読 | 査読あり |
| 標題 | Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo |
| 執筆形態 | 共著 |
| 掲載誌名 | INVESTIGATIVE OPHTHALMOLOGY&VISUAL SCIENCE |
| 掲載区分 | 国外 |
| 出版社・発行元 | ASSOC RESEARCH VISION OPHTHALMOLOGY INC |
| 巻・号・頁 | 49(1),pp.93-104 |
| 著者・共著者 | Yukiko Nakano,Masahito Oyamada,Ping Dai,Takuo Nakagami,Shigeru Kinoshita,Tetsuro Takamatsu |
| 概要 | PURPOSE. To explore connexin43 (Cx43) knockdown as an efficient treatment for corneal endothelial injury in an in vivo rat corneal scrape injury model.METHODS. Scrape injury was induced in the corneal endothelium, and immunolabeling (ZO-1, alpha-SMA, Cx43) was performed to analyze changes in Cx43 expression during wound healing. Single injection of Cx43 antisense oligodeoxynucleotide (AS-ODN), small interfering RNA (siRNA), or adenovirus (CMV-Cx43-mRFP1) was applied into the anterior chamber simultaneously with the injury, and wound closure was examined by immunolabeling (ZO-1, Cx43) and propidium iodide staining. Corneal endothelium proliferation on day 1 after injury was studied by Ki67-immunolabeling. Cx43-knockdown treatment was performed also without injury, and its effect on Cx43 expression and Ki67 immunolabeling was examined. |
| DOI | 10.1167/iovs.07-0255 |
| ISSN | 01460404 |
| PMID | 18172080 |
| PermalinkURL | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=39549089570&origin=inward |