オヤマダ マサヒト   Oyamada Masahito
  小山田 正人
   所属   藤女子大学  人間生活学部 食物栄養学科
   職種   特任教授
言語種別 英語
発行・発表の年月 1996/12
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 Differential regulation of gap junction protein (connexin) genes during cardiomyocytic differentiation of mouse embryonic stem cells in vitro
執筆形態 共著
掲載誌名 EXPERIMENTAL CELL RESEARCH
掲載区分国外
出版社・発行元 ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
巻・号・頁 229(2),pp.318-326
著者・共著者 Y Oyamada,K Komatsu,H Kimura,M Mori,M Oyamada
概要 Using an in vitro system for differentiation of embryonic stem (ES) cells into cardiac myocytes, we analyzed the expression of connexin (Cx) genes by RTPCR to learn what changes in the expression of multiple connexin genes occur during the early stage of heart development. We also examined gap junctional intercellular communication by using Lucifer Yellow dye microinjection transfer studied intracellular Ca2+ transients by confocal laser image analysis using flue 3, and determined localization of Cx43 by immunofluorescence during in vitro differentiation of ES cells into cardiac myocytes,
DOI 10.1006/excr.1996.0377
ISSN 00144827
PMID 8986615
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