ミツモト　ヤスヒデ   Yasuhide Mitsumoto
  光本　泰秀
   所属   薬学部 薬学科
   職種   教授
言語種別 英語
発行・発表の年月 1987/04
形態種別 学術論文
査読 査読あり
標題 Stimulation of leucine transport by a phorbol ester through activation of protein kinase C and Na+/H+ exchanger
執筆形態 共著
掲載誌名 Biochem Biophys Res Commun
掲載区分国外
巻・号・頁 144,pp.900-906
担当区分 筆頭著者
著者・共著者 Y Mitsumoto, T Mohri
概要 A synthetic diacylglycerol, 1-oleoyl-2-acetyl-sn-glycerol (OAG), as well as 12-O-tetradecanoylphorbol-13-acetate (TPA) has been found to elevate the cytoplasmic pH and increase leucine uptake dose-dependently, when added to quiescent cultures of Chang liver cell. Addition of either a protein kinase C inhibitor, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), or an Na+/H+ antiporter inhibitor, ethylisopropylamiloride (EIPA), abolished completely or incompletely the TPA-stimulated leucine uptake and TPA-induced cytoplasmic alkalinization. Therefore the stimulation of leucine uptake by OAG and TPA is proposed to be elicited at least partly through activation of Na+/H+ antiporter. We suggest that activation of protein kinase C by the phorbol ester is responsible for the stimulation of Na+/H+ exchange system and also leucine uptake in the cell.