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ヤマグチ ルイ
Rui Yamaguchi
山口 類 所属 熊本保健科学大学 保健科学部 医学検査学科 熊本保健科学大学大学院 保健科学研究科 保健科学専攻 職位 准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2016/01 |
| 形態種別 | 研究論文（学術雑誌） |
| 査読 | 査読あり |
| 標題 | Human neutrophil elastase induce IL-10 expression in PBMC through PKC
theta/delta and phospholipase pathways |
| 執筆形態 | 共著 |
| 掲載誌名 | Cell J. |
| 掲載区分 | 国外 |
| 巻・号・頁 | 17,pp.692-700 |
| 総ページ数 | 8 |
| 著者・共著者 | Kawata J, Yamaguchi R, Yamamoto T, Ishimaru Y, Sakamoto A, Aoki M, Kitano M, Umehashi M, Hirose E, Yamaguchi Y |
| 概要 | A phospholipase C inhibitor (U73122) partially blunt- ed the induction of IL-10 mRNA expression by HNE, while IL-10 mRNA expression was significantly reduced by a protein kinase C (PKC) inhibitor (Rottlerin). A calcium chelator (3,4,5-trimethoxybenzoic acid 8-(diethylamino)octyl ester: TMB-8) inhibited the response of IL-10 mRNA to stimulation by HNE. In addition, pretreatment with a broad-spectrum PKC inhibitor (Ro-318425) partly blocked the response to HNE. Finally, an inhibitor of PKC theta/delta abolished the increased level of IL-10 mRNA expression.These results indicate that HNE mainly upregulates IL-10 mRNA ex- pression and protein production in moncytes via a novel PKC theta/delta, although partially via the conventional PKC pathway. |