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ヤマグチ ルイ
Rui Yamaguchi
山口 類 所属 熊本保健科学大学 保健科学部 医学検査学科 熊本保健科学大学大学院 保健科学研究科 保健科学専攻 職位 准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2016/02 |
| 形態種別 | 記事・総説・解説・論説等（学術雑誌） |
| 査読 | 査読あり |
| 標題 | Halophilic metal binding protein and its His-Asp repeating peptides as fusion partner for high solubility-purification of fusion proteins |
| 執筆形態 | 共著 |
| 掲載誌名 | Bull Soc Sea Water Sci. |
| 掲載区分 | 国内 |
| 巻・号・頁 | 70,pp.51-52 |
| 総ページ数 | 1 |
| 担当区分 | 筆頭著者 |
| 著者・共著者 | Yamaguchi R, Ishibashi M, Tokunaga H, Arakawa T, Tokunaga M |
| 概要 | Histidine-rich metal binding protein（HP）has been found to have high binding capacity to several heavy metal ions and was expressed in Escherichia coli cytoplasm as soluble form in large amount. Here, we employed properties of this halophilic HP as a solubility-enhancing partner protein for soluble expression and for immobilized metal ion affinity purification. We also demonstrated that a novel insertion sequence（HD50）of HP containing His-Asp repeating sequence exhibited metal binding capacity. |