|
ヒルタ オサム
Osamu HIRUTA
蛭田 修 所属 熊本保健科学大学 品質保証・精度管理学共同研究講座 熊本保健科学大学大学院 保健科学研究科 保健科学専攻 職位 特命教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 2002 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Factors affecting the composition of oligosaccharides produced in chitosan hydrolysis using immobilized chitosanases |
| 執筆形態 | 共著 |
| 掲載誌名 | Biotechnology Progress |
| 掲載区分 | 国外 |
| 巻・号・頁 | 18(5),pp.969-974 |
| 総ページ数 | 6 |
| 著者・共著者 | Takashi Kuroiwa, Sosaku Ichikawa, Osamu Hiruta, Seigo Sato, Sukekuni Mukataka |
| 概要 | The hydrolysis reaction of chitosan using immobilized chitosanases with regard to the composition of its products and the yield of the intermediate target products, pentamer and hexamer of chitosan oligosaccharides, was investigated. Chitosanase was immobilized onto agar or agarose gel particles by the multipoint attachment method. In batch experiments, surface enzyme density, support particle size, temperature, agitator speed, and initial substrate concentration significantly affected the composition of the oligosaccharides produced. It was believed that these factors all related to the reaction rate and mass transfer rate at the surface of the support materials immobilizing the enzymes. These effects were summarized as a correlation with Damköhler number (Da), defined as the ratio of the maximum reaction rate to the maximum mass transfer rate. The result showed that the reaction conditions that give a low value of Da provide a high yield of pentamer and hexamer oligosaccharides. |