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ヤマグチ ルイ
Rui Yamaguchi
山口 類 所属 熊本保健科学大学 保健科学部 医学検査学科 熊本保健科学大学大学院 保健科学研究科 保健科学専攻 職位 准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2015/12 |
| 形態種別 | 記事・総説・解説・論説等（学術雑誌） |
| 査読 | 査読あり |
| 標題 | Halophilic starch-binding domain as a novel fusion protein partner for efficient recombinant protein expression |
| 執筆形態 | 共著 |
| 掲載誌名 | Bull Soc Sea Water Sci. |
| 掲載区分 | 国内 |
| 巻・号・頁 | 69,pp.384-385 |
| 総ページ数 | 1 |
| 著者・共著者 | Yamaguchi R, Ishibashi M, Tokunaga H, Arakawa T, Tokunaga M |
| 概要 | Halophiles produce halophilic enzymes which function well in high salinity conditions. The starch binding domain（SBD）of halophilic α-amylase has been found to show typical halophilic characteristics with high aqueous solubility and folding capacity. Here we developed a fusion protein expression vector, pSBF-S1, using this SBD as a fusion partner, looking for highly soluble protein expression and efficient affinity-purification. The fusion protein expressed could be purified by dual affinity chromatographies, i.e. Ni-affinity resin with N-terminal His-tag and amylose-resin with SBD affinity. The target protein is then generated through thrombin-digestion of fusion protein at the cleavage site between the target protein and SBD. |