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ヤマグチ ルイ
Rui Yamaguchi
山口 類 所属 熊本保健科学大学 保健科学部 医学検査学科 熊本保健科学大学大学院 保健科学研究科 保健科学専攻 職位 准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2012/01 |
| 形態種別 | 研究論文（学術雑誌） |
| 査読 | 査読あり |
| 標題 | Halophilic characterization of starch-binding domain from Kocuria varians alpha-amylase. |
| 執筆形態 | 共著 |
| 掲載誌名 | Int J Biol Macromol. |
| 掲載区分 | 国外 |
| 巻・号・頁 | 50,pp.95-102 |
| 総ページ数 | 7 |
| 担当区分 | 筆頭著者 |
| 著者・共著者 | Yamaguchi R, Inoue Y, Tokunaga H, Ishibashi M, Arakawa T, Sumitani J, Kawaguchi T, Tokunaga M |
| 概要 | The tandem starch-binding domains (KvSBD) located at carboxy-terminal region of halophilic α-amylase from moderate halophile, Kocuria varians, were expressed in E. coli with amino-terminal hexa-His-tag and purified to homogeneity. The recombinant KvSBD showed binding activity to raw starch granules at low to high salt concentrations. The binding activity of KvSBD to starch was fully reversible after heat-treatment at 85°C. Circular dichroism and thermal scanning experiments indicated that KvSBD showed fully reversible refolding upon cooling after complete melting at 70°C in the presence of 0.2-2.0M NaCl. The refolding rate was enhanced with higher salt concentration |