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サカモト チヨミ
SAKAMOTO Chiyomi
坂本 智代美 所属 熊本保健科学大学 生物毒素・抗毒素共同研究講座 職位 特命講師 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2017/10/15 |
| 形態種別 | 研究論文 |
| 標題 | Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ |
| 執筆形態 | 共著 |
| 掲載誌名 | Molecular Biology of the Cell |
| 掲載区分 | 国外 |
| 担当範囲 | Morphological quantification of chromatin and axes |
| 著者・共著者 | Ono T, Sakamoto C, Nakao M, Saitoh N, Hirano T |
| 概要 | we have established a set of two-step protocols for inducing reversible assembly of chromosome structure in situ, namely within a whole cell. This assay combined with small interfering RNA depletion demonstrates that the recovery of chromatin shapes and the reorganization of axes are highly sensitive to depletion of condensin II but less sensitive to depletion of condensin I or topoisomerase IIα. Furthermore, quantitative morphological analyses using the machine-learning algorithm wndchrm support the notion that chromosome shaping is tightly coupled to the reorganization of condensin II-based axes. We propose that condensin II makes a primary contribution to mitotic chromosome architecture and maintenance in human cells. |