|
ヒルタ オサム
Osamu HIRUTA
蛭田 修 所属 熊本保健科学大学 品質保証・精度管理学共同研究講座 熊本保健科学大学大学院 保健科学研究科 保健科学専攻 職位 特命教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 1988 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Production of 9-hydroperoxy-γ-linolenic acid by soybean lipoxygenase in a two-phase system |
| 執筆形態 | 共著 |
| 掲載誌名 | Journal of American Oil Chemical Society |
| 掲載区分 | 国外 |
| 出版社・発行元 | American Oil Chemical Society |
| 巻・号・頁 | 65,pp.1911 |
| 著者・共著者 | Osamu Hiruta, Toro Nakahara, Toshihiro Yokochi, Yashushi Kamisaka, Osamu Suzuki |
| 概要 | 9‐Hydroperoxy‐γ‐linolenic acid (9‐GOOH) was produced selectively by soybean lipoxygenase (LG) from γ‐linolenic acid (GLA) using a two‐phase (borate buffer, pH 6.5/hexane) system at a low temperature (10°C) with some anionic surfactants that showed little inhibitory effect on the enzyme at pH 6.5. The system avoided the inhibitory effect of a higher substrate concentration and of hydroperoxide as well as low substrate solubility in an aqueous system. Not lipoxygenase‐2 (LG2) but lipoxygenase‐1 (LG1) was indicated to be responsible for the production of 9‐GOOH. Among the anionic surfactants examined, acetate and sarcosinate were shown to be suitable, but phosphate was not. Ca2+ increased the 9‐GOOH productivity. The LG1 fraction gave the maximum yield of 35% with 0.5 mM Ca2+, ECT‐3N (anionic surfactant, acetate) at 10°C and at 4.8 mM GLA in an emulsion. |