アオキ ユウジ
Yuji.AOKI
青木 雄次
- 所属 松本大学大学院 健康科学研究科 健康科学専攻
- 松本大学 人間健康学部 健康栄養学科
- 職種 教授
| 言語種別 | 英語 |
| 発行・発表の年月 | 2000 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Reassessment of K-ras mutations at codon 12 by direct PCR and sequencing from tissue microdissection in human pancreatic adenocarcinomas |
| 執筆形態 | 共著 |
| 掲載誌名 | Pancreas |
| 掲載区分 | 国外 |
| 巻・号・頁 | 21,pp.152-157 |
| 総ページ数 | 8 |
| 著者・共著者 | Yuji Aoki, Shigetoshi Hosaka, Naoki Tachibana, Yasuyuki Karasawa, Shigeyuki Kawa, Kendo Kiyosawa |
| 概要 | The K-ras mutation status was reassessed by direct polymerase chain reaction (PCR) and sequencing from tissue microdissection without DNA extraction in 10 pancreatic carcinoma. Eight pancreatic adenocarcinomas were found to harbor K-ras mutations at codon 12 of either GTT or GAT. Mutations at the sites other than codon 12 were found in seven of 70 cloens by the TA cloning analysis. It was suggested that highly sensitive assay to detect the K-ras mutaions could cause artificial mutations. |