ナカムラ ケンスケ   KENSUKE NAKAMURA
  中村 建介
   所属   前橋工科大学  工学部 生命情報学科
   前橋工科大学大学院  工学研究科 生命情報学専攻(修士課程)
   前橋工科大学大学院  工学研究科 環境・生命工学専攻(博士課程)
   前橋工科大学  工学部 情報・生命工学群
   職種   教授
言語種別 英語
発行・発表の年月 2018
形態種別 研究論文 
査読 査読あり
標題 Variations in mating-type-like(MTL)loci direct PCR-based tracking of Zygosaccharomyces strains formed by mating
執筆形態 共著
掲載誌名 The Journal of general and applied microbiology
掲載区分国外
巻・号・頁 64(3),pp.127-135
著者・共著者 Tomoo Ogata, Katsuaki Kuroki, Koki Ito, Akifumi Kondo, Kensuke Nakamura
概要 Variations of chromosomal structures and nucleotide sequences around mating-type-like (MTL) loci among Zygosaccharomyces species have been reported. We have analyzed these differences in more detail and, on the basis of PCR-and next-generation sequencing data, we describe the MTL loci on chromosomes C and F for Z. rouxii type-strain NBRC1130, Z. rouxii NBRC0740 and Zygosaccharomyces sp. NBRC1876. We developed a mating strategy for Zygosaccharomyces sp. NBRC1876 and Z. rouxii NBRC0740, and found that the mated stains could be identified from parental strains on the basis of nucleotide sequence variations of the MTL loci. We further obtained evidence that Zygosaccharomyces sp. NBRC1876 is a natural interspecies hybrid between Z. rouxii and a related species.