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ミヤイ サヤカ
Sayaka Miyai
宮井 紗弥香 所属 動物看護学部 動物看護学科 職種 講師 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2008/04 |
| 形態種別 | 学術雑誌 |
| 標題 | Characterization of meFucoidan as a selective inhibitor for secretary phospho lipase A2-IIA and the Phosphorylation of meFucoidan-binding proteins by A-kinase in vitro |
| 執筆形態 | 共著 |
| 掲載誌名 | Biol. Pharm. Bull., 31(4), 714-718 (2008) |
| 著者・共著者 | Hiroko Maruyama, Kanzo Suzuki, Sayaka Miyai and Kenzo Ohtsuki |
| 概要 | The direct interaction of Mekabu fucoidan (meFucoidan) with four functional basic proteins (sPLA2-IIA, bFGF, histone H2B and HBV core protein) and three synthetic FGF-BP peptides (sp5, GE13 and RS6) was characterized in vitro. It was found that (i) meFucoidan inhibited dose-dependently the activity of sPLA2-IIA, but not pPLA2, through its direct binding to the enzyme; (ii) sPLA2-IIA activity was sensitive to meFucoidan rather than heparin, but significantly stimulated by sulfatide; (iii) the A-kinase-mediated phosphorylation of these basic proteins, except sPLA2-IIA, and synthetic peptides, containing potent phosphorylation sites for A-kinase, was inhibited dose-dependently by meFucoidan; and (iv) two consensus meFucoidan-binding motifs (B-B-B-B-X and B-X-B-B-X; B, basic amino acid) in these basic proteins and synthetic peptides could be overlapping to the potent phosphorylation site (B-B-X-S/T) for the kinase in vitro. |