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ミヤイ サヤカ
Sayaka Miyai
宮井 紗弥香 所属 動物看護学部 動物看護学科 職種 講師 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2010/12 |
| 形態種別 | 学術雑誌 |
| 標題 | Biochemical characterization of epigallocatechin-3-gallate as an effective stimulator for the GSK-3b-mediated phosphorylation of its binding proteins in vitro |
| 執筆形態 | 共著 |
| 掲載誌名 | Biol. Pharm. Bull., 33(12), 1932-1937 (2010) |
| 著者・共著者 | Sayaka Miyai, Akira Yamaguchi, Tetsumi Iwasaki, Fazel Shamsa and Kenzo Ohtsuki |
| 概要 | The stimulatory and inhibitory EGCG and its related two compounds (luteolin and quercetin) on the phosphorylation of four proteins [bMBP,hrTP,hrVM and rCRMP-2] by GSK-3βwere comparatively determined in vitro. We found that (i) EGCG, not quercetin and luteolin, highly stimulated the GSK-3β-mediated phosphorylation of hrTP and
significantly stimulated the phosphorylation of bMBP and hrVM by the kinase; (ii) these three polyphenols inhibited dose-dependently the phosphorylation of rCRMP-2 by GSK-3β; (iii) only EGCG significantly enhanced autophosphorylation of GSK-3β; and (iv) EGCG had a binding-affinity with two basic proteins (bMBP and hrTP) and a low affinity with rCRMP-2 rather than hrVM in vitro. In addition, the binding of EGCG to these two basic proteins induced to highly stimulate their phosphorylation, including novel potent sites for GSK-3β, and to significantly reduce the K(m) value and increase the V(max) value of these two substrate proteins for the kinase in vitro. |