Faculty Information - YOSHIKANE Yu

■ Present specialized field

Family and consumer sciences, and culture and living, Food sciences, Nutrition science and health science

■ Academic background

1.	2005/04～2008/03	〔Doctorial Course〕, Ehime University, Completed,
2.	2003/04～2005/03	〔Master Course〕, Kochi University, Completed,
3.	1999/04～2003/03	Kochi University, Graduated,

■ Business career

1.	2021/04～2023/03	Head of other organization
2.	2021/04～	Associate Professor
3.	2018/04～	Associate Professor

■ Book and thesis

1.	2024	Article	The health benefits of tofu, an unfermented soybean food that regulates the intestinal environment HSOA Journal of Food Science and Nutrition 10,pp.183; doi: 10.24966/FSN-1076/100183 (Collaboration)
2.	2022	Article	Health effects of drinking water produced from deep sea water: A randomized double-blind controlled trial Nutrients 14(3),pp.581 (Collaboration)
3.	2020	Article	Drinking refined deep-sea water improves the gut ecosystem with beneficial effects on the intestinal health in human: a randomised double-blind controlled trial Nutrients 12(9),pp.2646, doi:org/10.3390/nu12092646 (Collaboration)
4.	2019	Article	Determination of flavonoids and limonoids in yuzu seed oils and carrier oils Japan Journal of Aromatherapy pp.13-21 (Collaboration)
5.	2018	Article	Determination of furocoumarins concentrations in several essential oils and yuzu seed oils Japan Journal of Aromatherapy pp.1-9 (Collaboration)
6.	2015	Article	Inhibitory effects of carrier oils on mushroom tyrosinase and Melanogenesis Japan Journal of Aromatherapy pp.54-62 (Collaboration)
7.	2015	Article	Crystal structure of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD⁺-dependent dismutase from Mesorhizobium loti Biochemical and Biophysical Research Communications 456,pp.35-40 (Collaboration)
8.	2014	Article	Structure of 4-pyridoxolactonase from Mesorhizobium loti Acta Crystallographica Section F: Structural Biology and Crystallization Communications 70(4),pp.424-432 (Collaboration)
9.	2014	Article	Crystal structure of pyridoxine 4-oxidase from Mesorhizobium loti Biochimica et Biophysica Acta: Proteins and Proteomics 1834(6),pp.953-963 (Collaboration)
10.	2014	Article	The possibility of collaboration between university and local area in Human Resource Development program: a case study of Tosa Food Business Creator Collaboration, Journal of Collaborative Community Studies Unit Kochi Univ. pp.101-106 (Collaboration)
11.	2013	Article	Aroma-releasing potency and skin-lightening effect of yuzu (Citrus junos Sieb. ex Tanaka) seed oil as carrier oil Japan Journal of Aromatherapy pp.14-20 (Collaboration)
12.	2013	Article	Distribution of virulence markers among Vibrio vulnificus isolates of clinical and environmental origin and regional characteristics in Japan PLOS One 8(1),pp.e55219, doi:10.1371/journal.pone.0055219 (Collaboration)
13.	2012	Article	Properties of yuzu (Citrus junos Sieb. Ex Tanaka) seed oil as a carrier oil supporting aromatherapeutic application Japan Journal of Aromatherapy pp.41-49 (Collaboration)
14.	2012	Article	The mll6786 gene encodes a repressor protein controlling the degradation pathway for vitamin B₆ in Mesorhizobium loti FEMS Microbiology letters 329(2),pp.116-122 (Collaboration)
15.	2010	Article	Development of simultaneous enzymatic assay method for all six individual vitamin B₆ forms and pyridoxine-β-glucoside Journal of Nutritional Science and Vitaminology 56(3),pp.157-163 (Collaboration)
16.	2010	Article	Engineering Mesorhizobium loti pyridoxamine–pyruvate aminotransferase for production of pyridoxamine with L-glutamate as an amino donor Journal of Molecular Catalysis B 67(1-2),pp.104-110 (Collaboration)
17.	2010	Article	Crystallization and preliminary X-ray analysis of SDR-type pyridoxal dehydrogenase from Mesorhizobium loti Acta Crystallographica Section F: Structural Biology and Crystallization Communications 66(6),pp.718-720 (Collaboration)
18.	2009	Article	Crystallization and preliminary X-ray analysis of 4-pyridoxolactonase from Mesorhizobium loti Acta Crystallographica Section F: Structural Biology and Crystallization Communications 65(9),pp.886-889 (Collaboration)
19.	2009	Article	Crystallization and preliminary X-ray analysis of AAMS amidohydrolase, the final enzyme in degradation pathway I of pyridoxine Acta Crystallographica Section F: Structural Biology and Crystallization Communications 65(8),pp.829-831 (Collaboration)
20.	2009	Article	Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD⁺-dependent dismutase The Journal of Biochemistry 145(4),pp.493-503 (Collaboration)
21.	2008	Article	Synthesis of 4-pyridoxolactone from pyridoxine using a combination of transformed Escherichia coli cells Journal of Bioscience and Bioengineering 106(5),pp.460-465 (Collaboration)
22.	2008	Article	Gene identification and characterization of the pyridoxine degradative enzyme 4-pyridoxic acid dehydrogenase from the nitrogen-fixing symbiotic bacterium Mesorhizobium loti MAFF303099 The Journal of Biochemistry 143(5),pp.603-609 (Collaboration)
23.	2008	Article	Gene identification and characterization of the pyridoxine degradative enzyme α-(N-acetylaminomethylene)succinic acid amidohydrolase from Mesorhizobium loti MAFF303099 Journal of Nutritional Science and Vitaminology 54(3),pp.185-190 (Collaboration)
24.	2008	Article	Determination of individual vitamin B₆ compounds based on enzymatic conversion to 4-pyridoxolactone Journal of Nutritional Science and Vitaminology 54(1),pp.18-24 (Collaboration)
25.	2008	Article	Crystal structure of pyridoxamine-pyruvate aminotransferase from Mesorhizobium loti MAFF303099 The Journal of Biological Chemistry 283(2),pp.1120-1127 (Collaboration)
26.	2006	Article	Molecular cloning, identification and characterization of 2-methyl-3-hydroxypyridine-5-carboxylic-acid-dioxygenase-coding gene from the nitrogen-fixing symbiotic bacterium Mesorhizobium loti Journal of Bioscience and Bioengineering 102(6),pp.504-510 (Collaboration)
27.	2006	Article	Identification of a new tetrameric pyridoxal 4-dehydrogenase as the second enzyme in the degradation pathway for pyridoxine in a nitrogen-fixing symbiotic bacterium, Mesorhizobium loti Archives of Biochemistry and Biophysics 452(1),pp.1-8 (Collaboration)
28.	2006	Article	Molecular cloning, expression and characterization of pyridoxamine-pyruvate aminotransferase Biochemical Journal 396(3),pp.499-507 (Collaboration)
29.	2005	Article	Synthesis of 4-pyridoxolactone from pyridoxal with Escherichia coli cells expressing heterologous genes Biotechnology and Food Engineering pp.191-196 (Collaboration)
30.	2005	Article	4-Pyridoxolactonase from a symbiotic nitrogen-fixing bacterium Mesorhizobium loti: cloning, expression, and characterization Biochimica et Biophysica Acta: Proteins and Proteomics 1753(2),pp.234-239 (Collaboration)
31.	2004	Article	Crystallization and preliminary X-ray analysis of pyridoxal 4-dehydrogenase, the second enzyme in degradation pathway I of pyridoxine Acta Crystallographica Section D: Biological Crystallography 60(11),pp.2061-2062 (Collaboration)
32.	2004	Article	The nitrogen-fixing symbiotic bacterium Mesorhizobium loti has and expresses the gene encoding pyridoxine 4-oxidase involved in the degradation of vitamin B₆ FEMS Microbiology Letters. 234(2),pp.225-230 (Collaboration)
33.	2004	Article	Coenzyme precursor-assisted cooperative overexpression of an active pyridoxine 4-oxidase from Microbacterium luteolum Protein Expression and Purification 34(2),pp.243-248 (Collaboration)
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■ Academic conference presentation

1.	2020/02	Validation of the refined-deep-seawater for human health care by Kochi project based on Industry-academia-government platform in Japan (31st World Congress on Nutrition & Food Sciences)
2.	2019/03	Industry-academia-government collaboration project in Kochi, Japan: Application of the refined-deep-seawater for human health care (20th International Congress on Nutrition & Health 2019)
3.	2018/03	An improvement of the human gut ecosystem by drinking refined-deep-seawater (RDSW) (Euro Food 2018, 21st Euro-Global Summit on Food and Beverages 2018)
4.	2017/01	Human resources development program in a local food industry in Japan -Tosa FBC project- (VLAG International Course, Advanced Food Analysis)
5.	2014/08	Crystal structure of pyridoxine 4-oxidase from Mesorhizobium loti (Congress and General Assembly of the International Union of Crystallography)
6.	2012/08	Characteristics of yuzu seed oil and its application to aromatherapy (The 1st International Congress of Aromatherapy)
7.	2012/08	Inhibitory effects of German chamomile (whole plant) extract on tyrosinase activity (The 1st International Congress of Aromatherapy)
8.	2009/08	Topology structure and biochemical functions of 4-pyridoxic acid dehydrogenase in bacteria (21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology)
9.	2006/06	Active site structure of pyridoxamine-pyruvate aminotransferase (20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress)
10.	2005/11	Synthesis of 4-pyridoxolactone from pyridoxal with Escherichia coli cells expressing heterologous genes (Regional Symposium on Chemical Engineering)
11.	2005/11	Genes encoding enzymes involved in degradation for vitamin B₆ (International Interdisciplinary Conference on Vitamins, Coenzymes, and Biofactors)
12.	2005/11	Two types of pyridoxal 4-dehydrogenase in Mesorhizobium loti (International Interdisciplinary Conference on Vitamins, Coenzymes, and Biofactors)
13.	2005/11	A sequence and homology of pyridoxamine-pyruvate aminotransferase (International Interdisciplinary Conference on Vitamins, Coenzymes, and Biofactors)
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■ E-Mail Address